Device

Part:BBa_K3702206:Design

Designed by: Dat Vo, Christine Cremeans   Group: iGEM20_FSU   (2020-10-10)


Formaldehyde inducible ParD2 generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 405
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This device was designed to be part of a kill switch along with another generator producing the ParD2 antitoxin. The purpose of the formaldehyde inducible kill switch was to kill the host cell (E. coli) when the host cell get out of the wastewater treatment plant. This part is formaldehyde inducible making the host cell with the kill switch only surviving in present of formaldehyde. This part combined with the ParE2 generator (BBa_K3702192) and methane converter (BBa_K3702204,BBa_K3702203) creates a methane dependent kill switch, where the methane is converted to methanol and then to formaldehyde. The formaldehyde will then induce the ParD2 antitoxin generator which would bind to the ParE2 toxin. In absent of methane however, ParD2 Antitoxin will not be produced and the ParE2 Toxin will kill the host cell.

Source

https://parts.igem.org/Part:BBa_K2728001 https://parts.igem.org/Part:BBa_B0034 https://parts.igem.org/Part:BBa_K2150106 https://parts.igem.org/Part:BBa_B0015

References